Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.

摘要: Abstract A 2.2 kb cDNA clone which presumably includes the complete human B-raf coding sequence was isolated from a testes library. Sequence analysis showed presence of all three conserved regions CR1, CR2 and CR3 previously identified in raf protein kinases. The expressed E. coli yielding fusion reacts with antibodies specific for raised against C-terminal peptide as well monoclonal map into kinase domain.