Serumless medium for cultivation of cells of normal and malignant origin.

摘要: SummaryA serumless medium suitable for prolonged growth and repeated subculture of Walker carcinosarcoma 256, sarcoma 180, KB human heart cells in vitro was described. In addition to amino acids, vitamins, minerals glucose, it contained insulin, salmine sulfate, pyruvate, mucate, methyl oleate, folinic acid lactalysate. The latter group materials with exception mucate required maximal rates, comparable those on media containing dialyzed serum. Suspension cultures 256 were grown continuous passage the medium.