Deregulated Adhesion Program in Palatal Keratinocytes of Orofacial Cleft Patients.
作者： Mammadova , Carels , Zhou , Gilissen , Helmich
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摘要: Orofacial clefts (OFCs) are the most frequent craniofacial birth defects. An orofacial cleft (OFC) occurs as a result of deviations in palatogenesis. Cell proliferation, differentiation, adhesion, migration and apoptosis crucial We hypothesized that deregulation these processes oral keratinocytes contributes to OFC. performed microarray expression analysis on palatal from OFC non-OFC individuals. Principal component showed clear difference gene with 24% 17% for first second component, respectively. In cells, 228 genes were differentially expressed (p < 0.001). Gene ontology enrichment involved β1 integrin-mediated adhesion migration, well P-cadherin expression. A scratch assay demonstrated reduced (343.6 ± 29.62 μm) vs. (503.4 41.81 μm, p 0.05). Our results indicate deregulated keratinocytes, which might contribute pathogenesis.
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