Multi-tier regulation of the streptomycete morphogenetic peptide SapB.

摘要: Summary Streptomyces coelicolor is a morphologically complex bacterium requiring the secretion of surface-active proteins to progress through its life cycle. SapB represents an important class these biosurfactants, as illustrated by ability restore aerial hyphae formation when applied exogenously developmental mutants. However, such fail sporulate, exemplifying need co-ordinate timing production with other events. has unusual lantibiotic structure. Its structural gene, ramS, only 38 nucleotides downstream gene encoding putative modification enzyme, RamC. Transient, co-ordinated expression operon was thought be controlled response regulator RamR. we show that ramS transcribed throughout cell cycle dual profile dissimilar tightly ramC expression. Surprisingly, post-translational relies on prior membrane localization precursor peptide, RamS, demonstrated absence RamS in S. coelicolor treated Bacillus subtilis lipoprotein surfactin. Our results demonstrate interspecies interaction can also mediated interference Further, temporal and spatial regulation irreversible morphogenetic peptide suggests new model for control key