Fluorescent Dye Labeling Changes the Biodistribution of Tumor-Targeted Nanoparticles.

作者: Patricia Álamo , Victor Pallarès , María Virtudes Céspedes , Aïda Falgàs , Julieta M. Sanchez

DOI: 10.3390/PHARMACEUTICS12111004

关键词:

摘要: Fluorescent dye labeling is a common strategy to analyze the fate of administered nanoparticles in living organisms. However, which extent processes can alter original nanoparticle biodistribution has been so far neglected. In this work, two widely used fluorescent molecules, namely, ATTO488 (ATTO) and Sulfo-Cy5 (S-Cy5), have covalently attached well-characterized CXCR4-targeted self-assembling protein (known as T22-GFP-H6). The labeled T22-GFP-H6-ATTO T22-GFP-H6-S-Cy5 then compared that non-labeled different CXCR4+ tumor mouse models. We observed while parental T22-GFP-H6 accumulated mostly specifically cells, showed dramatic change pattern, accumulating non-target organs such liver or kidney reducing targeting capacity. Therefore, use molecules should be avoided target tissue uptake studies during design development targeted nanoscale drug delivery systems, since their effect over nanomaterial lead considerable miss-interpretations actual biodistribution.

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