Peptide retention prediction using hydrophilic interaction liquid chromatography coupled to mass spectrometry.
作者： Majors J. Badgett , Barry Boyes , Ron Orlando
DOI: 10.1016/J.CHROMA.2017.12.055
关键词:
摘要: A model that predicts retention for peptides using a HALO® penta-HILIC column and gradient elution was created. Coefficients each amino acid were derived linear regression analysis these coefficients can be summed to predict the of peptides. This has high correlation between experimental predicted times (0.946), which is on par with previous RP HILIC models. External validation performed set H. pylori samples same LC-MS system used create model, deviation from actual low. Apart composition, length location residues peptide examined two site-specific corrections hydrophobic at N-terminus as well one spot over
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