Affinity chromatography and affinity partition of human serum pre-albumin using immobilized remazol yellow GGL: Evidence that albumin increases binding of pre-albumin to the dye
作者： G. Birkenmeier , B. Tschechonien , G. Kopperschläger
DOI: 10.1016/0014-5793(84)81097-2
关键词:
摘要: Pre-albumin was isolated from human serum or plasma by ammonium sulphate fractionation and chromatography on dye-substituted Sephadex G-100 to a high degree of purity. The interaction the pre-albumin with dye Remazol Yellow GGL studied in more detail means affinity partitioning an aqueous two-phase system composed dextran poly(ethylene glycol). In presence glycol) partition coefficient increases, indicating dye. Human albumin found enhance this considerably. A similar effect also shown bovine but not egg
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