Phage susceptibility testing and infectious titer determination through wide-field lensless monitoring of phage plaque growth.

摘要: The growing number of drug-resistant bacterial infections worldwide is driving renewed interest in phage therapy. Based on the use a personalized cocktail composed highly specific viruses, this therapy relies range tests agar media to determine most active given target (phage susceptibility testing), or isolate new lytic phages from an environmental sample (enrichment banks). However, these culture-based techniques are still solely interpreted through direct visual detection plaques. main objective work investigate computer-assisted methods order ease and accelerate diagnosis but also study plaque growth kinetics. For purpose, we designed custom wide-field lensless imaging device, which allows continuous monitoring over very large area sensor (3.3 cm2). Here report Staphylococcus aureus 3 hr estimation infectious titer 8 20 min. These much shorter time-to-results than 12 24 hours traditionally needed, since naked eye observation counting plaques widely used technique for testing prior Moreover, samples enables kinetics, deeper understanding interaction between bacteria. Finally, thanks 4.3 μm resolution, detect phage-resistant microcolonies Klebsiella pneumoniae inside boundaries thus show that our prototype suitable device track resistance. Lensless therefore all-in-one method could easily be implemented cost-effective compact devices laboratories help with diagnosis.