A strategy to analyse activity-based profiling of tyrosine kinase substrates in OCT-embedded lung cancer tissue.

摘要: The use of optimal cutting temperature (OCT) medium has served to improve the long-term preservation surgical tissue specimens. Unfortunately, presence polymers in OCT been found generate signal interference proteomic-based techniques. Indeed lysates precludes analysis activity based proteomic profiles obtained from lung adenocarcinoma (LuAdCa) resection In order probe this question further were prepared 47 non-neoplastic and tumour, node, metastasis (TNM) stage 1 LuAdCa specimens embedded with or without OCT, data multiplex protein tyrosine kinase peptide substrates obtained. We that changes overall phosphorylation level coincided subsequently developed an per median correcting strategy by performing centering on values each peptide. Application post-analytical not only can identify but also assist identifying novel targets for therapeutic intervention against LuAdCa.