High density lipoprotein is the major carrier of lipid hydroperoxides in human blood plasma from fasting donors.

摘要: Abstract Analysis of untreated fresh blood plasma from healthy, fasting donors revealed that high density lipoprotein (HDL) particles carry most (approximately 85%) the detectable oxidized core lipids. Low (LDL) lipids are relatively peroxide-free. In vitro mild oxidation gel-filtered with a low, steady flux aqueous peroxyl radicals initially caused preferential HDL rather than LDL until ubiquinol-10 present in was consumed. Thereafter, were more rapidly. Isolated lipoproteins behaved similarly. Preferential accumulation lipid hydroperoxides reflects lack antioxidants compared to LDL, which contained 8-12 alpha-tocopherol and 0.5-1.0 molecules per particle. Cholesteryl ester (CEOOHs) stable when added at 37 degrees C for up 20 hr. Transfer CEOOHs too slow have influenced data. Incubation mildly cultured hepatocytes afforded linear removal (40% loss over 1 hr), whereas fast-then-slow biphasic observed HDL. Our data show is principal vehicle circulating suggest may be rapidly those vivo. The rapid hepatic clearance could imply possible beneficial role by attenuating build-up LDL.