Brain Region-Specific Expression of MeCP2 Isoforms Correlates with DNA Methylation within Mecp2 Regulatory Elements

摘要: MeCP2 is a critical epigenetic regulator in brain and its abnormal expression or compromised function leads to spectrum of neurological disorders including Rett Syndrome autism. Altered the two isoforms, MeCP2E1 MeCP2E2 has been implicated complications. However, expression, regulation functions isoforms are largely uncharacterized. Previously, we showed role neuronal maturation reported as major protein isoform adult mouse brain, embryonic neurons astrocytes. Recently, that DNA methylation at regulatory elements (REs) within Mecp2 promoter intron 1 impact differentiating neural stem cells. This current study aimed for comparative analysis temporal, regional cell type-specific developing brain. displayed later onset than during development. In female male hippocampus, both were detected neurons, astrocytes oligodendrocytes. Furthermore, was relatively uniform different regions (olfactory bulb, striatum, cortex, thalamus, brainstem cerebellum), whereas differential enrichment these regions. Both similar distribution regions, except cerebellum. Lastly, preferential correlation observed between specific CpG dinucleotides REs isoform-specific Taken together, show display patterns development may this Mecp2/MeCP2 Our results significantly contribute towards characterizing profiles thereby provide insights on potential