The raspberry locus encodes Drosophila inosine monophosphate dehydrogenase.

摘要: Investigation of an enhancer-trap line exhibiting testis-specific beta-galactosidase expression led to the isolation Drosophila gene encoding inosine monophosphate dehydrogenase (IMPD), rate-limiting enzyme in guanine nucleotide synthesis, which has been implicated cell cycle control and malignant transformation. Northern situ hybridization analysis demonstrated that a complex pattern involving several independently regulated transcripts. Two ubiquitous, but highly ovary enriched, transcripts 2.5 1.9 kb are expressed nurse cells delivered oocyte, whilst 0.9 transcript is found exclusively testis. The encodes 58 kDa protein, similar length sequence mouse human IMPDs presumably required for GTP synthesis during early embryogenesis. Over-expression this cDNA Escherichia coli yielded product predicted size, was possess IMPD activity spectrophotometric assay. coding capacity other currently uncertain. We present evidence raspberry (ras) locus at 9E functions discussed relation phenotypes ras mutants.