Species-specific transcription in mice carrying human chromosome 21.

摘要: Homologous sets of transcription factors direct conserved tissue-specific gene expression, yet factor-binding events diverge rapidly between closely related species. We used hepatocytes from an aneuploid mouse strain carrying human chromosome 21 to determine, on a chromosomal scale, whether interspecies differences in transcriptional regulation are primarily directed by genetic sequence or nuclear environment. Virtually all locations, landmarks initiation, and the resulting expression observed were recapitulated across entire hepatocyte nucleus. Thus, homologous tissues, is largely responsible for directing programs; epigenetic machinery, cellular environment, themselves play secondary roles.