Circulating microRNAs for predicting and monitoring response to mechanical circulatory support from a left ventricular assist device

摘要: Aims There are few non-invasive techniques to predict and monitor patients' responses left ventricular assist device (LVAD) therapy. MicroRNAs (miRs) small non-coding RNAs with intricate roles in cardiovascular disease. They stable the circulation, readily quantified, may be useful as new biomarkers. This study sought identify candidate miR biomarkers for further investigation. Methods results We studied 53 plasma 20 myocardial samples from 19 patients who underwent HeartMate II LVAD implantation, used a screening microarray analyse change expression of 1113 miRs after 6 months support. Twelve showed significant variation validation, yielding miR-1202 miR-483-3p In test cohort, circulating early sustained up-regulation support, median (interquartile range) fold changes baseline 2.17 (1.43–2.62; P = 0.011), 2.27 (1.12–2.42; P = 0.036), 1.87 (1.64–4.36; P = 0.028), 2.82 (0.70–10.62; P = 0.249) at 3, 6, 9, 12 months, respectively, whilst identified good vs. poor responders [absolute 1.296 (1.293–1.306) 1.311 (1.310–1.318) arbitrary units; P = 0.004]. Both enriched myocardium, suggesting heart possible source fraction. Conclusions This is first report patients. We demonstrate feasibility this approach, potential miR-1202, response therapy, propose work these hypotheses elucidate clinical practice underlying biological processes.