Isolation and Characterization of PHA-Producing Bacteria from Propylene Oxide Saponification Wastewater Residual Sludge

摘要: A polyhydroxyalkanoate (PHA)-producing strain was isolated from propylene oxide (PO) saponification wastewater activated sludge and identified as Brevundimonas vesicularis UJN1 through 16S rDNA sequencing Biolog microbiological identification. Single-factor response surface methodology experiments were used to optimize the culture medium conditions. The optimal C/N ratio 100/1.04, carbon nitrogen sources sucrose (10 g/L) NH4Cl (0.104 g/L) respectively. conditions consisted of initial pH 6.7 an incubation temperature 33.4 °C for 48 h, with 15% inoculum 100 mL at agitation rate 180 rpm. PHA concentration reached 34.1% cell dry weight increased three times compared that before optimization. only report PHA-producing bacteria by showed conversion PHAs using glucose source 1.67%. In our research, 3.05%, production much cheaper than source.