Use of a channel biosensor for the assay of paralytic shellfish toxins
作者： Byeung Soo Cheun , Michael Loughran , Tetsuhito Hayashi , Yuji Nagashima , Etsuo Watanabe
DOI: 10.1016/S0041-0101(98)00015-4
关键词:
摘要: Gonyautoxin (GTX), saxitoxin (STX) and tetrodotoxin (TTX), also known as paralytic shellfish poisons (PSP), block Na+ channels, including those in the frog bladder membrane. A tissue biosensor has been developed, consisting of a electrode covered with membrane integrated within flow cell. The direction transfer, investigated absence channel blockers, established that active transport occurs across frogs from internal to external face. Transfer was shown be TTX sensitive. sensor response each different PSP recorded results compared toxicities determined by standard mouse bio-assay. Using high concentrations puffer fish Takifugu niphobles, linear correlation found between two assay systems. However, system able detect very low samples species (Takifugu niphobles pardalis) at below detection limit
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