Genetic Analysis of Arabidopsis Mutants

摘要: INTRODUCTIONThe inheritance of mutant phenotypes in Arabidopsis thaliana is most often analyzed the progeny resulting from crosses between different parents. This article describes genetic strategies for analysis mutants. Newly identified mutations can be examined initially using segregation analysis, followed by backcrossing and cosegregation removal extraneous assessment pleiotropy. Confirmation that a phenotype interest results given mutation achieved via complementation testing, which also used to determine allelism recessive mutations. The construction double mutants useful study signal transduction metabolic pathways. Considerations use homozygotes their identification F(2) F(3) generations are described at end this article.