The thalamic mGluR1-PLCβ4 pathway is critical in sleep architecture.

摘要: The transition from wakefulness to a nonrapid eye movement (NREM) sleep state at the onset of involves low-voltage, high-frequency irregular electroencephalography (EEG) waveforms large-amplitude, low-frequency EEG accompanying synchronized oscillatory activity in thalamocortical circuit. circuit consists reciprocal connections between thalamus and cortex. cortex sends strong excitatory feedback thalamus, however function which is unclear. In this study, we investigated role thalamic metabotropic glutamate receptor 1 (mGluR1)-phospholipase C β4 (PLCβ4) pathway control PLCβ4-deficient (PLCβ4−/−) mice. mGluR1-PLCβ4 contains synapses that receive corticothalamic inputs. PLCβ4−/− mice, NREM was stimulated, stabilized, resulted increased sleep. power density delta (δ) waves parallel with These phenotypes mice were consistent TC-restricted PLCβ4 knockdown Moreover, vitro intrathalamic oscillations greatly enhanced slices. results our study showed critical controlling architecture.