Proteomic changes driven by urban pollution suggest particulate matter as a deregulator of energy metabolism, mitochondrial activity, and oxidative pathways in the rat brain.

摘要: The adverse effects of air pollution have been long studied in the lung and respiratory systems, but molecular changes that this causes at central nervous system level yet to be fully investigated understood. To explore evolution with time protein expression levels brain rats exposed particulate matter different sizes, we carried out two-dimensional gel electrophoresis followed by determination dysregulated proteins through Coomassie blue staining-based densities (SameSpots software) subsequent identification using MALDI-based mass spectrometry. Expression differences were found statistically significant p-value <0.05. A systems biology-based approach was utilized determine critical biochemical pathways involved rats' response. Our results suggest brains a size dependent-response, being mitochondrial activity astrocyte function severely affected. proteomic study confirms dysregulation involving energy metabolism, activity, oxidative as some main PM exposure on rat brain. SIGNIFICANCE: Rat origin car engines are affected two areas: many linked chain, neuronal astrocytic function, which stimulates triggering tumorigenesis neurodegeneration.