Accurate assessment of HER2 gene status for invasive component of breast cancer by combination of immunohistochemistry and chromogenic In Situ hybridization

摘要: The specimens of ductal carcinoma in situ (DCIS) with early invasion, and collected by core needle biopsy (CNB) tend to contain limited amount invasive component, so it is imperative explore a new technique which can assess HER2 gene status accurately for the cancer component these specimens. Dual staining combining immunohistochemistry (IHC) myoepithelial cells single or dual probe chromogenic hybridization (CISH) was performed on routinely processed paraffin sections from 20 cases diagnosed as having DCIS cancer. Among them, 10 had fluorescence (FISH)-confirmed amplification FISH-confirmed non-amplification HER2. We successfully detected genetic signals IHC markers (SMM-HC CK5/6) simultaneously section all Myoepithelial assay were consistent those individual alone. results determined 100% FISH. allows simultaneous detection marker protein cancerous feasible, has potential be used clinical practice effective determination component.