Artificial Skin – Culturing of Different Skin Cell Lines for Generating an Artificial Skin Substitute on Cross-Weaved Spider Silk Fibres

摘要: Background: In the field of Plastic Reconstructive Surgery development new innovative matrices for skin repair is in urgent need. The ideal biomaterial should promote attachment, proliferation and growth cells. Additionally, it degrade an appropriate time period without releasing harmful substances, but not exert a pathological immune response. Spider dragline silk from Nephila spp meets these demands to large extent. Methodology/Principal Findings: Native spider silk, harvested directly out spiders, was woven on steel frames. Constructs were sterilized seeded with fibroblasts. After two weeks cultivating single fibroblasts, keratinocytes added generate bilayered model, consisting dermis epidermis equivalents. For next three weeks, constructs co-culture lifted originally designed setup air/liquid interface cultivation. culturing period, embedded paraffin especially developed program spidersilk avoid supercontraction. Paraffin cross- sections stained Haematoxylin & Eosin (H&E) microscopic analyses. Conclusion/Significance: frames provides suitable matrix 3 dimensional cell culturing. Both fibroblasts lines adhere fibres proliferate. Guided by fibres, they sprout into meshes reach confluence at most one week. A well-balanced, cocultivation continuously separated strata can be achieved serum reduction, changing medium conditions cultivation interphase. Therefore appears promising enhancement regeneration.