Protein diversity in discrete structures at the distal tip of the trypanosome flagellum.

摘要: The distal end of the eukaryotic flagellum/cilium is important for axonemal growth and signaling has distinct biomechanical properties. Specific flagellum tip structures exist, yet their composition, dynamics, functions are largely unknown. We used biochemical approaches to identify seven constituents flagella connector at an assembling trypanosome three capping structure tips both mature flagella. Both contain evolutionarily conserved as well kinetoplastid-specific proteins, component assembly into occurs very early during extension. Localization functional studies reveal that membrane junction attached extending microtubules by a kinesin-15 family member. On opposite side, kinesin facilitates attachment in flagellum. Functional also suggest roles several other components definition subdomains structures.