EFFECT OF METHYLGLYOXAL ON PROTEIN THIOL AND AMINO GROUPS IN ISOLATED RAT ENTEROCYTES AND COLONOCYTES AND ACTIVITY OF VARIOUS BRUSH BORDER ENZYMES

摘要: The effect of methylglyoxal on protein -SH and -NH2 groups in cytosolic membranous fractions epithelial cells lining the gastrointestinal tract rat was investigated, using isolated villus crypt (enterocytes) colonocytes. It found that 11-12% 14-17% membrane were lost when treated with 2 mM methylglyoxal. In colonocytes, corresponding loss 46 30% under same treatment. Similarly, 27-37% group fraction 18-19% enterocytes by 30 15% fractions, respectively, Effect activity various brush border enzymes such as alkaline phosphatase, gamma-glutamyl transpeptidase, leucine aminopeptidase, Mg2(+)-ATPase, sucrase lactase also studied. Alkaline phosphatase transpeptidase activities inhibited to extent respectively. There no significant change other after treating vesicles These findings show can cause thiol amino enzyme mucosal is more pronounced which are constant contact bacterial metabolites.