Biodegradation of Phthalate Isomers by Pseudomonas aeruginosa PP4, Pseudomonas sp. PPD and Acinetobacter lwoffii ISP4

摘要: Pseudomonas aeruginosa PP4, sp. PPD and Acinetobacter lwoffii ISP4 capable of utilizing phthalate isomers were isolated from the soil using enrichment culture technique. The strain metabolizes isophthalate, while PP4 utilizes all three (ortho-, iso- tere-) as sole carbon source. isophthalate (0.1%) more rapidly (doubling time, 0.9 h) compared to (4.64 h), (7.91 h) other reported strains so far. metabolic pathways in these isolates initiated by dihydroxylation isomers. Phthalate is hydroxylated 3,4-dihydro-3,4-dihydroxyphthalate 4,5-dihydro-4,5-dihydroxyphthalate PPD, respectively; terephthalate 2-hydro-1,2-dihydroxyterephthalate. All hydroxylate 4-hydro-3,4-dihydroxyisophthalate. generated dihydroxyphthalates subsequently metabolized 3,4-dihydroxybenzoate (3,4-DHB) which was further ortho ring-cleavage pathway. cells grown on phthalate, or showed respiration respective isomer activity corresponding ring-hydroxylating dioxygenase, suggesting source specific induction different dioxygenases. We report, for first dioxygenase its reductase component cell-free extracts. enzyme maximum with reduced nicotinamide adenine dinucleotide (NADH) pH range 8–8.5. Cells glucose failed respire 3,4-DHB significantly low activities enzymes that are inducible.