Chromosomal characterization of methotrexate-resistant human T-lymphoblast leukemia cells (CCRF-CEM) with impaired polyglutamylation.

摘要: Two sublines of the human T-lymphoblast leukemia cell line CCRF-CEM, which were resistant to methotrexate (MTX) due defective MTX polyglutamate synthesis, karyologically characterized. No statistically significant differences in modal number chromosomes noted cells (CCRF-CEM/P) as compared parent (91, range, 86-123; and 93, range; 78-103, respectively). Fifteen marker identified their origins at least partially established. An isochromosome 7q, (marker 13) was present all MTX-resistant but not found any sensitive karyotype. This chromosome may be involved emergence drug-resistant from parental population CCRF-CEM cells. In lines, 8, 9 14 appear highly unstable are genesis many chromosomes. These also implicated vivo various leukemias lymphomas, suggests that both tumor progression vitro cellular adaptation marked by mutations activate multiple oncogenes.