Manure removal system influences the abundance and composition of airborne biotic contaminants in swine confinement buildings

摘要: Little is known about the factors influencing abundance and community composition of airborne biotic contaminants in swine confinement buildings (SCBs). Microbial air samples were collected from three different SCBs equipped with types manure removal systems (deep-pit slats, scraper system, deep-litter bed system). The all analyzed using cultivation-independent methods. V1–V3 region 16S rRNA gene was amplified extracted DNA sequenced 454-pyrosequencing. abundances genes six tetracycline resistance (tetB, tetH, tetZ, tetO, tetQ, tetW) quantified real-time PCR. significantly higher a deep-pit system except for tetB gene. This contrasts opposite trend found previously by culture-based studies. aerial bacterial composition, as measured pairwise Bray–Curtis distances, varied according to system. rRNA-based pyrosequencing revealed Firmicutes (72.4 %) dominant group Lactobacillus major genus, while Actinobacteria constituted 10.7 % detectable bacteria. more abundant deep pit whereas highly Overall, results this study suggest that plays key role structuring SCBs.