Kinetic validation of 6-NBDG as a probe for the glucose transporter GLUT1 in astrocytes.

作者: Luis Felipe Barros , Carla X. Bittner , Anitsi Loaiza , Iván Ruminot , Valeria Larenas

DOI: 10.1111/J.1471-4159.2009.05885.X

关键词:

摘要: In recent years, the use of fluorescent glucose analogs has allowed study rapid transport modulation in heterogeneous cell cultures and complex tissues. However, kinetic behavior these tracers is not conventional. For instance, analog 6-NBDG permeates 50-100 times slower than but uptake almost insensitive to glucose, an observation that casts doubts as specificity pathway. To investigate this apparent anomaly cultured astrocytes, which are rich transporter GLUT1, we first estimated parameters uptake, were then incorporated into model GLUT1. The main outcome analysis was binds GLUT1 with 300 higher affinity explains why its efficiently displaced by glucose. high binding also cytochalasin B less effective at inhibiting uptake. We conclude 6-NBDG, used low concentrations, astrocytes chiefly through advise exofacial inhibitor 4,6-ethylidine-D-glucose be used, instead tool choice confirm

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