Impact of surface structure and feed gas composition on Bacillus subtilis endospore inactivation during direct plasma treatment
作者： Christian Hertwig , Veronika Steins , Kai Reineke , Antje Rademacher , Michael Klocke
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摘要: This study investigated the inactivation efficiency of cold atmospheric pressure plasma treatment on Bacillus subtilis endospores dependent used feed gas composition and surface, were attached on. Glass petri-dishes, glass beads, peppercorns inoculated with same endospore density treated a radio frequency jet. Generated reactive species detected using optical emission spectroscopy. A quantitative polymerase chain reaction (qPCR) based ratio detection system was established to monitor DNA damage during treatment. Argon + 0.135% vol. oxygen 0.2% nitrogen as emitted highest amounts UV-C photons considerable amount species. Plasma generated argon characterized by (ROS), whereas negligible. The use pure showed negligible UV atomic oxygen, however, vacuum (V)UV assumed. Similar maximum results achieved for three compositions. surface structure had significant impact between 2.4 2.8 log10 petri-dishes 3.9 4.6 beads. resulted in an lower than 1.0 log10. qPCR all Pure values, followed nitrogen. In case seems be dominated action ROS. These findings indicate role VUV process B. endospores.
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