Reduced DNA Repair Capacity in Breast Cancer Patients and Unaffected Individuals from Breast Cancer Families

摘要: Abstract It has been suggested that increased fragile site expression in lymphocyte cultures can be used as a marker for genetic predisposition to cancer. We wished determine whether aphidicolin (APC), an inhibitor of the DNA repair enzyme polymerase α, could reliable biomarker identification capacity unaffected individuals at high risk from breast cancer families. PHA-stimulated cultures, with and without APC, were set up 65 individuals, whom 14 patients, 26 families, 25 controls. A significant proportion patients familial (FBC) families exhibited premature separation centromeres (PSC) aneuploidy untreated cultures. In APC treated almost all such marked depression mitotic index aneuploidy, compared Our results indicate these have defective capacity. Such thus much higher persons exhibiting PSC or defects alone. propose may valuable identifying FBC