Characterization of new cristamonad species from kalotermitid termites including a novel genus, Runanympha.

作者: Rudolf H Scheffrahn , Martin Kolisko , Martin Kolisko , Vittorio Boscaro , Patrick J Keeling

DOI: 10.1038/S41598-021-86645-W

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摘要: Cristamonadea is a large class of parabasalian protists that reside in the hindguts wood-feeding insects, where they play an essential role digestion lignocellulose. This group symbionts boasts impressive array complex morphological characteristics, many which have evolved multiple times independently. However, their diversity understudied and molecular data remain scarce. Here we describe seven new species cristamonad from Comatermes, Calcaritermes, Rugitermes termites Peru Ecuador. To classify these species, examined cells by light scanning electron microscopy, sequenced symbiont small subunit ribosomal RNA (rRNA) genes, carried out barcoding mitochondrial rRNA gene hosts to confirm host identification. Based on data, five characterized here represent within described genera: Devescovina sapara n. sp., aymara Macrotrichomonas ashaninka secoya yanesha sp. Additionally, two with overall characteristics similar poorly-studied probably polyphyletic 'joeniid' Parabasalia are classified genus Runanympha gen.: illapa pacha

参考文章(36)
R.H. Scheffrahn, A.J. Mullins, J. Krecek, Chase, J.A, J.R. Mangold, T. Myles, T. Nishimura, R. Setter, R.A. Cannings, R.J. Higgins, Lindgren B.S., R Constantino, S. R., Issa, E. and Kuswanto, Global elevational, latitudinal, and climatic limits for termites and the redescription of Rugitermes laticollis Snyder (Isoptera: Kalotermitidae) from the Andean Highlands Sociobiology. ,vol. 62, pp. 426- 438 ,(2015) , 10.13102/SOCIOBIOLOGY.V62I3.793
S. Tamm, S.L. Tamm, Rotary movements and fluid membranes in termite flagellates. Journal of Cell Science. ,vol. 20, pp. 619- 638 ,(1976) , 10.1242/JCS.20.3.619
CHRISTOPHE NOËL, SATOKO NODA, CLEA MANTINI, MICHAEL F. DOLAN, SHIGEHARU MORIYA, PILAR DELGADO-VISCOGLIOSI, TOSHIAKI KUDO, MONIQUE CAPRON, RAYMOND J. PIERCE, MORIYA OHKUMA, ERIC VISCOGLIOSI, Molecular Phylogenetic Position of the Genera Stephanonympha and Caduceia (Parabasalia) Inferred from Nuclear Small Subunit rRNA Gene Sequences Journal of Eukaryotic Microbiology. ,vol. 54, pp. 93- 99 ,(2007) , 10.1111/J.1550-7408.2006.00234.X
Linda Medlin, Hille J. Elwood, Shawn Stickel, Mitchell L. Sogin, The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene. ,vol. 71, pp. 491- 499 ,(1988) , 10.1016/0378-1119(88)90066-2
Erick R. James, Fabien Burki, J. Todd Harper, Rudolf H. Scheffrahn, Patrick J. Keeling, Molecular Characterization of Parabasalian Symbionts Coronympha clevelandii and Trichonympha subquasilla from the Hawaiian Lowland Tree Termite Incisitermes immigrans Journal of Eukaryotic Microbiology. ,vol. 60, pp. 313- 316 ,(2013) , 10.1111/JEU.12027
L. R. Cleveland, Symbiosis between Termites and Their Intestinal Protozoa Proceedings of the National Academy of Sciences of the United States of America. ,vol. 9, pp. 424- 428 ,(1923) , 10.1073/PNAS.9.12.424
Ugo d’Ambrosio, Michael Dolan, Andrew M. Wier, Lynn Margulis, Devescovinid trichomonad with axostyle-based rotary motor (“Rubberneckia”): Taxonomic assignment as Caduceia versatilis sp. nov. European Journal of Protistology. ,vol. 35, pp. 327- 337 ,(1999) , 10.1016/S0932-4739(99)80011-X