Identification of a BamHI Polymorphism for the Urokinase Gene Associated with Symptomatic Coronary Artery Disease.

摘要: Urokinase-type plasminogen activator (u-PA) and plasmin have been implicated in a number of processes, including activation variety metalloproteinases, matrix remodeling, cell migration, which may underlie the early initiation progression atherosclerosis coronary artery disease (CAD). These studies were carried out to determine whether variations u-PA gene, using BamHI restriction fragment length polymorphism (RFLP) as new marker for genetic variation, be associated with CAD. Southern blot analysis individual digested genomic DNA (BamHI), hybridized 2-kb human cDNA probe, identified two-allele RFLP allelic bands at 6 1.5 kb. A constant band 9 kb was detected. Three genotypes designated 1/1 (6.0-kb only), 1/2 (6.0 1.5-kb bands), 2/2(1.5-kb only). For these studies, 43, umbilical cord samples, representing "control" population, analyzed compared terms their 34 saphenous vein samples from patients requiring bypass grafting (CABG). Controls, presumed reflect normal population distribution, showed genotype distribution 1/1(n = 8, 18.6%), (n 33, 76.7%) 2/2 2, 4.7%), whereas CAD 16, 47.1%), 13, 38.2%), 5, 14.7%). Comparison data derived CABG demonstrated significant difference P 0.002), an increased prevalence homozygous patients. demonstrate association between gene presence or absence