trans-resveratrol protects embryonic mesencephalic cells from tert-butyl hydroperoxide: electron paramagnetic resonance spin trapping evidence for a radical scavenging mechanism.
作者： Jenny Karlsson , Mia Emgard , Patrik Brundin , Mark J. Burkitt
DOI: 10.1046/J.1471-4159.2000.0750141.X
关键词:
摘要: Abstract : In recent years, the antioxidant and other pharmacological properties of resveratrol, a natural product present in grapes wine, have attracted considerable interest from biomedical research community. an examination potential neuroprotective compound, we investigated ability resveratrol to protect rat embryonic mesencephalic tissue, rich dopaminergic neurones, prooxidant tert-butyl hydroperoxide. Using electron paramagnetic resonance (EPR) spin-trapping technique, main radicals detected cell suspensions were tert-butoxyl radical methyl radical, indicating one-electron reduction peroxide followed by β-scission reaction. The appearance EPR signals trapped preceded onset cytotoxicity, which was almost exclusively necrotic nature. inclusion incubations resulted marked protection cells parallel experiments, able demonstrate scavenging involved direct competition between spin trap for reaction with radicals. To our knowledge, this is first example cytoprotection has been demonstrated kinetics be due its responsible toxicity prooxidant.
sci-hub.se 参考文章(49)
L Bavaresco, C Fregoni, E Cantù, M Trevisan, Stilbene compounds: from the grapevine to wine. Drugs Under Experimental and Clinical Research. ,vol. 25, pp. 57- 63 ,(1999)
Patrik Brundin, Jenny Karlsson, Mia Emgård, Gabriele S. Kaminski Schierle, Oskar Hansson, Åsa Petersén, Roger F. Castilho, Improving the survival of grafted dopaminergic neurons: a review over current approaches. Cell Transplantation. ,vol. 9, pp. 179- 195 ,(2000) , 10.1177/096368970000900205
V O'Donnell, M J Burkitt, Mitochondrial metabolism of a hydroperoxide to free radicals in human endothelial cells: an electron spin resonance spin-trapping investigation. Biochemical Journal. ,vol. 304, pp. 707- 713 ,(1994) , 10.1042/BJ3040707
Axel Schwekendiek, Gerda Pfeffer, Helmut Kindl, Pine stilbene synthase cDNA, a tool for probing environmental stress. FEBS Letters. ,vol. 301, pp. 41- 44 ,(1992) , 10.1016/0014-5793(92)80206-V
Cecil R. Pace-Asciak, Susan Hahn, Eleftherios P. Diamandis, George Soleas, David M. Goldberg, The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoid synthesis: implications for protection against coronary heart disease. Clinica Chimica Acta. ,vol. 235, pp. 207- 219 ,(1995) , 10.1016/0009-8981(95)06045-1
N. Nakao, E. M. Frodl, W. M. Duan, H. Widner, P. Brundin, Lazaroids improve the survival of grafted rat embryonic dopamine neurons. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 12408- 12412 ,(1994) , 10.1073/PNAS.91.26.12408
Hitoshi Taniguchi, Keith P. Madden, An in situ radiolysis time-resolved ESR study of the kinetics of spin trapping by 5,5-dimethyl-1-pyrroline-N-oxide Journal of the American Chemical Society. ,vol. 121, pp. 11875- 11879 ,(1999) , 10.1021/JA993140L
Mariarosaria Miloso, Alberto A.E Bertelli, Gabriella Nicolini, Giovanni Tredici, Resveratrol-induced activation of the mitogen-activated protein kinases, ERK1 and ERK2, in human neuroblastoma SH-SY5Y cells. Neuroscience Letters. ,vol. 264, pp. 141- 144 ,(1999) , 10.1016/S0304-3940(99)00194-9
Keisuke Makino, Takuya Hagiwara, Akifumi Hagi, Masatoshi Nishi, Akira Murakami, Cautionary note for DMPO spin trapping in the presence of iron ion. Biochemical and Biophysical Research Communications. ,vol. 172, pp. 1073- 1080 ,(1990) , 10.1016/0006-291X(90)91556-8
Eli Finkelstein, Gerald M. Rosen, Elmer J. Rauckman, Spin trapping. Kinetics of the reaction of superoxide and hydroxyl radicals with nitrones Journal of the American Chemical Society. ,vol. 102, pp. 4994- 4999 ,(1980) , 10.1021/JA00535A029