Applicability of Passive Sampling to Bioanalytical Screening of Bioaccumulative Chemicals in Marine Wildlife

摘要: Quantification of bioaccumulative contaminants in biota is time and cost-intensive the required extensive cleanup steps make it selective toward targeted chemical groups. Therefore tissue extracts prepared for analysis are not amenable to assess combined effects unresolved complex mixtures. Passive equilibrium sampling with polydimethylsiloxane (PDMS) has potential unbiased mixtures, PDMS can be directly dosed into cell-based bioassays. The passive approach was tested by exposing lipid-rich (dugong blubber; 85% lipid) spiked a known mixture hydrophobic (five congeners tetra- octachloro-dibenzo-p-dioxins). attained within 24 h. Lipid-PDMS partition coefficients (Klip-PDMS) ranged from 20 38, were independent hydrophobicity, range those previously measured organochlorine compounds. To test if bioanalysis without need cleanup, blubber-PDMS CAFLUX bioassay, which specifically targets dioxin-like chemicals. Small quantities lipids coextracted found affect kinetics regularly applied 24-h bioassay; however, this effect eliminated longer exposure period (72 h). validated method 11 unspiked dugong blubber samples (native) dioxin concentrations. These results provide first proof concept linking bioassays, could further extended other lipid rich species wider bioanalytical end points.