Collagen VI Regulates Normal and Transformed Mesenchymal Cell Proliferationin Vitro

摘要: Abstract Suggestions exist that, in addition to traditional growth factors, the extracellular matrix (ECM) of a cell can regulate its proliferation. This hypothesis was investigated with normal and transformed fibroblasts because they exhibit specific intracellular responses after adherence ECM produce large quantities proteins. Although cells cultured on different proteins grew more rapidly than those plastic, an individual protein were not correlated. To test if stimulate growth, soluble given plating. In this culture system only collagen VI (CVI), at concentration 20 μg/ml medium, increased 3T3 number 402% control by 72 h. Similar increases human HT 1080 numbers noted. DNA synthesis all three types 24 h CVI. A mixture CVI single chains, yielded reduction alkylation, stimulatory. However, efficiently inhibited induced native Antibody inhibition studies showed that region stimulating proliferation differs from site bound integrin receptor α2β1, which mediates adhesion immobilized Heparin portion CVI-induced These data demonstrate mesenchymal via pathway is independent α2β1 stimulatory appears be within helical collagen.