Simple and Accurate HPTLC-Densitometric Method for Quantification of Delafloxacin (A Novel Fluoroquinolone Antibiotic) in Plasma Samples: Application to Pharmacokinetic Study in Rats

摘要: Delafloxacin (DLX) is a recently-approved fluoroquinolone antibiotic, which recommended for the treatment of "acute bacterial skin and structure infections". A thorough literature survey revealed only single published method estimation DLX using UPLC-MS/MS technique in biological samples. There no high-performance thin-layer chromatography (HPTLC) has been reported dosage forms and/or Therefore, selective, sensitive, rapid validated HPTLC-densitometry used human plasma first time. HPTLC quantification internal standard (IS; gatifloxacin) was carried out on glass coated silica gel 60 F254 plates ternary mixture ethyl acetate:methanol:ammonia solution 5:4:2 (%, v/v/v) as mobile phase. Densitometric detection done at 344 nm. The Rf values were recorded 0.43 0.27 IS, respectively. linearity range obtained 16-400 ng/band. simple protein precipitation extraction analyte from methanol. proposed "linearity, accuracy, precision, robustness". successfully utilized assessment pharmacokinetic profile rats after oral administration. After administration, peak concentration 194.19 ng/ml 1 h. could be applied study therapeutic drug monitoring clinical practice.