INFLUENCE OF SOLVENT ON YIELD, COMPOSITION OF EXTRACTED COMPUNDS AND ANTIOXIDANT ACTIVITY OF PROPOLIS EXTRACTS

摘要: Propolis, a multifunctional material produced by bees, is one of the richest natural sources flavonoids and other phenolic antioxidants. In this work we have investigated yield, composition (total phenol flavonoid content) antiradical activity (DPPH radical scavenging) propolis extracts prepared using different mixture solvents (ethanol, acetone, water ethyl acetate). For each solvent mixture, three independent extractions were performed. The results show that increase lipophilicity in extraction yield. amount total polyphenols decreases linearly with (r2= 0.93 0.97 for ethanol respectively). content remains relatively constant at higher concentration rises steeply when aceton or reaches 80%. It interesting to note addition small acetate water/ethanol significant phenols extract. Radical scavenging activity, assayed DPPH free radical, was dependent on HPLC analysis has revealed presence several acids such as p-coumaric acid, pinocembrine galangine, which could explain strong properties extracts. used strongly affects chemical