Reduced Radiation Damage in Transmission Electron Microscopy of Proteins in Graphene Liquid Cells.

摘要: Liquid-phase electron microscopy (LPEM) is capable of imaging native (unstained) protein structure in liquid, but the achievable spatial resolution limited by radiation damage. This damaging effect more pronounced when targeting small molecular features than for larger structures. The matter even complicated because critical dose that a sample can endure before damage not only varies between proteins also critically depends on experimental conditions. Here, we examined beam observed optimized conditions using liquid enclosure assembled from graphene sheets. It has been shown reduce electrons biological materials. We used sensitive microtubule and investigated these structures as function frequencies with transmission (TEM). Microtubule samples were examin...