Insect-Induced Daidzein, Formononetin and Their Conjugates in Soybean Leaves

摘要: In response to attack by bacterial pathogens, soybean (Gylcine max) leaves accumulate isoflavone aglucones, glucosides, and glyceollins. contrast the dynamics of related insect-inducible metabolites in remain poorly understood. this study, we analyzed biochemical responses Spodoptera litura (Lepidoptera: Noctuidae) herbivory also S. gut contents, which contain oral secretion elicitors. Following herbivory, displayed an induced accumulation flavone aglycones 4’,7-dihyroxyflavone, daidzein, formononetin, glucoside daidzin. Interestingly, foliar application secretions elicited (daidzein formononetin), 7-O-glucosides (daidzin, ononin), 7-O-(6’-O-malonyl-β-glucosides) (malonyldaidzin, malonylononin). Consistent with up-regulation isoflavonoid biosynthetic pathway, folair phenylalanine levels increased following treatment. To establish that these metabolitic changes were result de novo biosynthesis, demonstrated labeled (13C9) was incorporated into aglucones. These results are consistent presence defense elicitors secretions. We demonstrate conjugates leaves, not only pathogens as previously demonstrated, but insect herbivory.