Radioimmunoassay of Drugs in Body Fluids in a Forensic Context

摘要: Radioimmunoassay (RIA) is a versatile and extremely sensitive technique that can detect low levels of large or small molecules in biological fluids with little no sample preparation. It therefore very useful forensic toxicology for screening body drugs abuse. This account describes the theory practice RIA particular reference to analysis context. outlined from basic principles but inherent assumptions are often inapplicable so empirical design an assay considered detail Particular emphasis given development assays drug classes structurally related compounds rather than individual drugs. The preparation radiolabeled drugs, synthesis immunogens raising anitisera, production polyclonal monoclonal antisera, methods separating free antibody-bound antigens reviewed. Quality assurance, trouble-shooting possible hazards offorensic discussed, published tabulated. Many non-isotopic immunoassays have been developed recent years omitted this because date they less applicable samples such as haemolysed blood frequently encountered toxicology. Future progress likely be concerned applying more compounds, improving preparing investigating use anti-drug antibodies.