A sample preparation technique using biocompatible composites for biomedical applications

摘要: Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential sensitive diagnostics. In this study, cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated support efficient situ nucleic acid preparation from enriched pathogens cells. CB introduced enhance rate effectiveness pathogen absorption using CB–DA composite. This novel achieved capture efficiency approximately 90% an Escherichia coli concentration 106 CFU/mL within 3 min. Real-time PCR analyses DNA samples recovered system showed four-fold increase early amplification signal strength, effective method capturing might be useful preparing diagnostic systems.