Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities.

摘要: PU.1 is a member of the ets family transcription factors and expressed exclusively in cells hematopoietic lineage. Mice homozygous for disruption DNA binding domain are born alive but die severe septicemia within 48 h. The analysis these neonates revealed lack mature macrophages, neutrophils, B T cells, although erythrocytes megakaryocytes were present. absence lymphoid commitment development null mice was not absolute, since maintained on antibiotics began to develop normal appearing 3-5 days after birth. In contrast, remained undetectable older mice. Within myeloid lineage, despite macrophages antibiotic-treated animals, few with characteristics neutrophils appear by day 3. While protein appears be essential lineage commitment, it absolutely required differentiation macrophages.