A Simple and Reliable HPTLC Method for the Determination of Four Marker Components in the Quality Control of Alstonia scholaris
作者： Anupam Maurya , Santosh Srivastava , None
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摘要: A new, simple, reliable, and validated high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitation of four bioactive markers, ursolic acid (1), betulinic (2), β-sitosterol (3), lupeol (4) in stem root barks Alstonia scholaris. Extraction efficiency targeted markers from bark matrixes with organic solvents using cold percolation, hot extraction, ultrasonic extraction were studied, which showed that was best sample preparation. The separation achieved on silica gel 60F254 HPTLC plates chloroform-methanol (99:1 v/v) as mobile phase. carried out densitometric reflection/absorption mode at 680 nm after post derivatization vanillin-sulphuric reagent. linear regression analysis data calibration plots a good relationship (r2 ≥ 0.998) concentration range 2–10 μg per spot a...
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