作者: Yuzhen Lu , Kitt G. Payn , Piyush Pandey , Juan J. Acosta , Austin J. Heine
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摘要: Abstract. Loblolly pine (Pinus taeda L.) is a commercially important timber species that planted across wide temperature gradient in the southeastern United States. Ensuring planting stock suitably adapted to growing environment critical achieve high productivity and survival. Mean winter minimum (MMT) an climatic variable related growth survival, used guide transfer of improved seed throughout distribution. Advanced generation families are assigned cold hardiness zones based on MMT region from which their founding ancestors were adapted. This method has been successful, but as number cycles breeding program increases, so too will increase for given selection. The precise assignment correct zone new selection become more challenging, particularly when originate varying geographic regions with range MMTs. Long term field studies, although considered most reliable identifying cold-tolerant families, extremely resource intensive time consuming. development high-throughput screening tool characterize quantify freeze tolerance among different genetic entries seedlings facilitate accurate deployment highly productive well-adapted loblolly landscape. study presents novel approach assess damage using hyperspectral imaging. A seedling population, comprising 98 representing at origin, was raised nursery. Using chamber, total 1549 subjected artificial mid-winter freeze. custom-assembled image system scanning before after event periodically. data processing pipeline developed segment extract spectra individual seedlings. Examination spectral features suggested reductions chlorophylls water concentrations freeze-susceptible Cost-sensitive linear support vector machine (SVM) utilized classifying visually scored into stressed healthy. Results showed imaging able geometric classification accuracies 75-78% non-symptomatic within 10 days event, up 96% day 41 event. top portion found be indicative events than middle bottom portions. newly evaluation provide breeders valuable offers efficiency objectivity characterizing potentially other resistance attributes pine.