Substitutions in the N-glycan core as regulators of biorecognition: the case of core-fucose and bisecting GlcNAc moieties.

摘要: Core fucosylation and the bisecting N-acetylglucosamine residue are prominent natural substitutions of N-glycan core. To address issue whether these two substituents can modulate ligand properties complex-type biantennary N-glycans, we performed chemoenzymatic synthesis respective galactosylated α2,3/6-sialylated N-glycans. Neoglycoproteins were then produced to determine glycans' reactivities with sugar receptors in solid-phase assays tumor cells vitro as well their vivo biodistribution profiles mice. Slight protein-type-dependent changes noted lectin binding, including adhesion/growth-regulatory galectins study objects, when data related N-glycans without or only one core substituent. Monitoring binding revealed cell-type-dependent changes. They delimited activity this glycan type from that chains un- monosubstituted cores. A markedly prolonged serum half-life was conferred to...