Highly Specific and Ultrasensitive Two-Photon Fluorescence Imaging of Native HOCl in Lysosomes and Tissues Based on Thiocarbamate Derivatives

摘要: Hypochlorous acid (HOCl) acts as a weak distributed mainly in acidic organelle lysosomes of phagocytes and plays crucial role the immune defense. The elaborate interrelation between variations HOCl levels different physiological pathological processes remains unclear. Thus, accurate determination lysosomal living cells vivo is very important. Because extremely low concentration difficulty distinguishing from OCl– under environment, it still great challenge to specifically monitor intracellular intrinsic without exogenous stimulation, which impedes an exact understanding its biological roles. In this paper, based on electrophilic addition Cl+ sulfide moiety, we have developed two-photon fluorescent probe O-(N-butyl-1,8-naphthalimide)-4-yl-N,N-dimethylthiocarbamate (NDMTC) for specific over other bioactive molecules. Our results show that NDMTC p...