Fractalkine-Induced Endothelial Cell Migration Requires MAP Kinase Signaling
作者： Michael V. Volin , Nha Huynh , Karolina Klosowska , Rosemary D. Reyes , James M. Woods
DOI: 10.1159/000272949
关键词:
摘要: Background/Aims: Angiogenesis is a well-established char- acteristic in the rheumatoid arthritis (RA) synovial pannus. We have previously demonstrated that fractalkine (Fkn/ CX3CL1) expression significantly increased RA joint and induces angiogenesis. In this work we studied mechanisms through which Fkn functions as an an- giogenic mediator. Methods: Human microvascular endo- thelial cells (HMVECs) human umbilical vein endothelial (HUVECs) were stimulated with analyzed by Western blotting or stained Alexa Fluor 488 phalloidin for F-actin to characterize time frame of cytoskeletal re- arrangement. Fkn-induced HUVEC chemotaxis was per- formed presence absence MAP kinase inhibi- tors. Results: Phalloidin staining revealed signifi- cant rearrangements HUVECs HMVECs starting early 10 min after stimulation. HMVEC stimulation 1-30 resulted phosphorylation JNK. also significant Erk 1/2 over course ranging from 1 15 min. A some- what similar (5-15 min) detected HMVECs. Inhibitors either JNK
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