High glucose enhances intracellular Ca2+ responses triggered by purinergic stimulation in retinal neurons and microglia.

摘要: Abstract Activation of purinergic P2 receptors, which are expressed in neurons and microglial cells, normally induces an increase intracellular calcium concentration ([Ca 2+ ] i ) some the inflammatory mediators excitatory neurotransmitters found to be implicated neuronal cell death observed diabetic retinas released response [Ca . However, it is unknown whether hyperglycemia/high glucose has effect changes triggered by activation receptors retinal cells. Using single-cell imaging studies, we that activation, both were potentiated cells had been cultured high conditions. In was mostly due Ca influx through voltage sensitive channels, whereas occurred mainly P2X receptor while there also a smaller component rise dependent on release from stores, probably P2Y activation. conclusion, our results show rat neural conditions increased responses This augmented might account for and, therefore, responsible early stages retinopathy.