Fatty Acid Profiling During Microbial Lipid Production Under Varying pO2 and Impeller Tip Speeds

摘要: The fatty acid profile study was undertaken to the effect of impeller tip speed-associated shear stress and dissolved oxygen saturation (DO) on composition variation total lipid content cells. in a 5-l stirred tank bioreactor using Mucor sp. RRL001. To interaction parameters their effects, central composite design used. profiling during course suggested that oleic palmitic were two major components with varying between 34–47% 29–39.1%, respectively, content. GLA varied 3% 9% lipid. also revealed presence minor amount acids chain length C:12, C:20, C:22, C:24. modeling accumulation it follows quadratic model both speed (p = 0.0166) concentration 0.0098) following order effect. fermenter run based optimum production zone response surface plot resulted maximum 4.8 g l−1 compared model-predicted value 4.49 l−1. present suggests is more significant contributor accumulation. However, fungal not directly associated or availability