Metabolomic profiling reveals differential effects of glucagon-like peptide-1 and insulin on nutrient partitioning in ovine liver.

摘要: This study was conducted to identify the insulin-independent actions of glucagon-like peptide-1 (GLP-1 (7-36 amide)) in partitioning nutrient metabolism ovine liver. Four Suffolk wethers (60.0 ± 6.7 kg body weight (BW)) were used a repeated-measure design under euglycemic--hyperinsulinemic and hyper -GLP-1 clamps for 150 min with intravenous infusion insulin (0.5 mU/kg BW/min; from 0 90 min), GLP-1 (0.5 µg/kg 60 150 min) both hormones co-administered 90 min. Liver biopsies collected at 0, 60, 90 150 min represent metabolomic profiling baseline, insulin, plus GLP-1, respectively, analyzed metabolites using Capillary Electrophoresis Time-of-Flight Mass Spectrometer. Metabolomics analysis reveals 51 as being significantly altered (P < 0.05) by compared baseline values. Insulin enhanced glycolysis, lipogenesis, oxidative stress defense cell proliferation pathways, but reduced protein breakdown, gluconeogenesis ketogenesis pathways. Conversely, promoted lipolytic ketogenic pathways accompanied lowered lipid clearance liver well elevated nucleotide degradation. Despite further research still warranted, our data suggest that may exert insulin-antagonistic effects on hepatic ruminants.