BT-07MEASURING TUMOR METABOLISM IN DIFFUSE INTRINSIC PONTINE GIOMAS (DIPG) USING HYPERPOLARIZED CARBON-13 MR SPECTROSCOPIC IMAGING

摘要: Diffuse intrinsic pontine gliomas (DIPGs) continue to have a very poor prognosis with median survival rate of 9 months. The methods for assessing treatment response and tumor progression are based on radiographic but conventional MRI is not sufficient predicting clinical outcome. A recent first-in-human study using hyperpolarized 13C MR metabolic imaging showed the safety feasibility this technology evaluating pyruvate metabolism in humans. purpose was demonstrate [1-13C]pyruvate as non-invasive tool evaluate vivo orthotopic brainstem xenografts injected human DIPG cells. Primary derived cells were implanted into athymic rats (n = 4). 3D magnetic resonance spectroscopic (MRSI) data acquired 3T scanner custom-designed 1H/13C coil after injection solution. MRSI exhibited an abnormal T2 lesions. mean normalized lactate, ratio lactate hyperintense voxels 3.1 ± 1.7, 1.5 0.4 0.47 0.2 (mean standard deviation), respectively, significantly higher than corresponding values from contra-lateral hemisphere. signal theses areas highly elevated compared normal appearing brain tissue supratentorial region. metabolite quantification revealed differentiation between core, periphery H&E stained slides viable that recapitulated histopathology subset high-grade pediatric astrocytomas. results suggest technique may provide unique will be beneficial management patients DIPG.